Device

Part:BBa_K4461502:Design

Designed by: SHIH-HSUN, YANG   Group: iGEM22_NYCU-Taipei   (2022-10-10)


nirBDC+RBS+mCherry+BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned the promoter from E.coli K-12 MG1655 by PCR. Then we used PCR again to add suffix and prefix. Also, we seperated the plasmid that we want to insert the promoter with two parts. At last, we used Gibson Assembly to ligate three fragments.



Source

gene synthesis

References